Long S K, Patrick R
Appl Microbiol. 1965 Nov;13(6):973-6. doi: 10.1128/am.13.6.973-976.1965.
The conditions required for production of levo 2,3-butylene glycol by Bacillus polymyxa from citrus molasses were studied. Starter cultures required acclimatization to the substrate prior to inoculation of the fermentation medium. Maximal production of butylene glycol with minimal residual sugar was obtained with a medium consisting of molasses, diluted to 20 degrees Brix, and 0.4% urea. Optimal environmental conditions included aeration at 0.11 volumes of air per volume of medium per minute, maintenance of pH at 6.0 to 6.2, a fermentation temperature of 30 C, and a stirring rate of 420 rev/min. The concentration of butylene glycol obtained in the fermentation beer ranged from 2.3 to 4.4%. The optical rotation of the glycol ranged from alpha (23 degrees ) = -1.01 degrees to -10.45 degrees . The variation in rotation was probably due to the presence of contaminating substances in the distillate.
研究了多粘芽孢杆菌利用柑橘糖蜜生产左旋2,3-丁二醇所需的条件。起始培养物在接种发酵培养基之前需要适应底物。使用由稀释至20波美度的糖蜜和0.4%尿素组成的培养基，可获得丁二醇产量最高且残留糖最少的结果。最佳环境条件包括：通气量为每分钟每体积培养基0.11体积空气，pH维持在6.0至6.2，发酵温度为30℃，搅拌速度为420转/分钟。发酵液中丁二醇的浓度范围为2.3%至4.4%。二醇的旋光度范围为α(23℃)= -1.01°至 -10.45°。旋光度的变化可能是由于馏出物中存在污染物。
