David J R, Paterson P Y
J Exp Med. 1965 Dec 1;122(6):1161-71. doi: 10.1084/jem.122.6.1161.
Peritoneal cells obtained from animals exhibiting delayed hypersensitivity are inhibited from migrating in vitro by specific sensitizing antigen. This test for the detection of delayed hypersensitivity was applied to the problem of cellular sensitivity in allergic encephalomyelitis (AE). The migration of peritoneal cells obtained from guinea pigs with AE was inhibited specifically by nervous tissue antigens. The specificity of this reaction was further studied. Neonatal rat nervous tissue, which was shown to lack the encephalitogenic antigen, i.e. did not produce AE when injected with complete adjuvant into guinea pigs and rats, did not inhibit the migration of cells from animals with AE. Adult rat nervous tissue, which readily produces AE, and thus contains the encephalitogenic antigen did inhibit the migration of such cells. The finding that cells from animals with AE display hypersensitivity which appears to be directed specifically to the encephalitogenic antigen strongly supports the view that such cells could play an important role in the pathogenesis of this disease.
从表现出迟发型超敏反应的动物身上获取的腹膜细胞，在体外会被特异性致敏抗原抑制迁移。这种用于检测迟发型超敏反应的试验被应用于变应性脑脊髓炎（AE）的细胞敏感性问题。患有AE的豚鼠的腹膜细胞迁移被神经组织抗原特异性抑制。对该反应的特异性进行了进一步研究。新生大鼠神经组织被证明缺乏致脑炎抗原，即当与完全佐剂一起注射到豚鼠和大鼠体内时不会产生AE，它不会抑制患有AE的动物的细胞迁移。容易产生AE并因此含有致脑炎抗原的成年大鼠神经组织确实会抑制此类细胞的迁移。患有AE的动物的细胞表现出似乎特异性针对致脑炎抗原的超敏反应这一发现，有力地支持了这样一种观点，即此类细胞可能在该疾病的发病机制中起重要作用。
