Banville R R
J Bacteriol. 1964 May;87(5):1192-7. doi: 10.1128/jb.87.5.1192-1197.1964.
Banville, Robert R. (The Catholic University of America, Washington, D.C.). Factors affecting growth of Staphylococcus aureus L forms on semidefined medium. J. Bacteriol. 87:1192-1197. 1964.-A semidefined agar medium was found suitable for production and cultivation of the L form of Staphylococcus aureus. In semidefined liquid medium, growth of the L form took place in the form of a sediment containing large masses of cells, but heavy and diffuse growth occurred in the same medium with 0.05% agar. The optimal pH for L-colony formation on solid medium was 6.5. More L colonies developed on 0.75% agar than at higher agar concentrations. L colonies developed in greater numbers on pour plates than on streak plates, and in some cases more L colonies appeared under anaerobic incubation. L-colony formation appeared to be inhibited by sodium citrate. The vitamin requirements of the L forms studied were similar to those of the classical form.
班维尔，罗伯特·R.（美国天主教大学，华盛顿特区）。影响金黄色葡萄球菌L型在半合成培养基上生长的因素。《细菌学杂志》87:1192 - 1197。1964年。——发现一种半合成琼脂培养基适合金黄色葡萄球菌L型的产生和培养。在半合成液体培养基中，L型以含有大量细胞的沉淀物形式生长，但在含有0.05%琼脂的相同培养基中出现大量且弥散的生长。在固体培养基上形成L菌落的最适pH值为6.5。在0.75%琼脂上形成的L菌落比在更高琼脂浓度下更多。倾注平板上形成的L菌落比划线平板上更多，并且在某些情况下，厌氧培养下出现的L菌落更多。柠檬酸钠似乎抑制L菌落的形成。所研究的L型的维生素需求与典型形式相似。
