甲氨蝶呤放射免疫测定法的开发与应用
Development and application of a radioimmunoassay for methotrexate.
作者信息
Aherne G W, Piall E M, Marks V
出版信息
Br J Cancer. 1977 Nov;36(5):608-17. doi: 10.1038/bjc.1977.238.
Abstract
An antiserum to methotrexate has been produced in a sheep against a conjugate of ovalbumin and methotrexate (MTX) prepared using a water-soluble carbodiimide. The antibodies produced were specific for substances containing the 2,4-diamino pteridine structure. Naturally occurring folates did not interfere with the assay. A radioimmunoassay has been developed using this antiserum, which can be used to measure MTX concentrations of less than 1 ng/ml in biological samples without prior extraction. The concentrations of MTX in the blood and urine of patients following a single i.v. bolus injection and following oral administration of the drug have been measured. The published radioimmunoassays for MTX have been compared.
摘要
通过使用水溶性碳二亚胺制备的卵清蛋白与甲氨蝶呤（MTX）的偶联物，在绵羊体内产生了抗甲氨蝶呤抗血清。所产生的抗体对含有2,4 - 二氨基蝶啶结构的物质具有特异性。天然存在的叶酸不干扰该测定。利用这种抗血清开发了一种放射免疫测定法，可用于在无需预先提取的情况下测量生物样品中甲氨蝶呤浓度低于1 ng/ml的情况。已测量了患者单次静脉推注和口服该药物后血液和尿液中甲氨蝶呤的浓度。并对已发表的甲氨蝶呤放射免疫测定法进行了比较。
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本文引用的文献
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