Takeuchi Y, Hisanaga N
Br J Ind Med. 1977 Nov;34(4):314-24. doi: 10.1136/oem.34.4.314.
Workers exposed to toluene develop many central and autonomic nervous symptoms. It has been suggested that the effects of toluene on the central nervous system may be detectable by EEG. In the present experiments, changes in EEG and behaviour of rats exposed to toluene were monitored in an attempt to clarify the relationship between exposure to toluene and central nervous system reaction. Chronically implanted electrodes were used in Wistar albino male rats to record EEG in cortex and hippocampus, cervical EEG and pulse rate. The rats were exposed to 4000 ppm, 2000 ppm and 1000 ppm toluene vapour for four hours. The sleep cycle was divided into five phases (wakeful, spindle, slow-wave, preparadoxical and paradoxical) judged by the cortical and hippocampal EEGs, the cervical EMG, and behaviour. This classification should be useful in assessment of the effects of toluene on the central nervous system. In our experiments, the changes in the sleep cycle suggest that 4000 ppm and 2000 ppm of toluene vapour disturb the sleep, and 1000 ppm of toluene vapour prevents sleep entering the slow-wave phase but facilitates its entry into the paradoxical phase. The changes of EEG components were peculiar to each concentration. The results suggest, in effect, that analysis of the changes in the EEG components would be helpful as an index to the reaction of the central nervous system to toluene vapour.
接触甲苯的工人会出现许多中枢和自主神经症状。有人提出，脑电图（EEG）或许能够检测出甲苯对中枢神经系统的影响。在本实验中，对接触甲苯的大鼠的脑电图和行为变化进行了监测，以阐明接触甲苯与中枢神经系统反应之间的关系。在雄性Wistar白化大鼠身上使用长期植入电极来记录大脑皮层和海马体的脑电图、颈部脑电图以及脉搏率。将大鼠暴露于4000 ppm、2000 ppm和1000 ppm的甲苯蒸汽中4小时。根据大脑皮层和海马体的脑电图、颈部肌电图以及行为，将睡眠周期分为五个阶段（清醒、纺锤波、慢波、前异相和异相）。这种分类对于评估甲苯对中枢神经系统的影响应该是有用的。在我们的实验中，睡眠周期的变化表明，4000 ppm和2000 ppm的甲苯蒸汽会干扰睡眠，而1000 ppm的甲苯蒸汽会阻止睡眠进入慢波阶段，但有助于进入异相阶段。脑电图成分的变化因每种浓度而异。实际上，结果表明，分析脑电图成分的变化将有助于作为中枢神经系统对甲苯蒸汽反应的指标。
