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小球藻中细胞色素f和质体蓝素的动力学。I. 闪光后的氧化动力学。

Cytochrome f and plastocyanin kinetics in Chlorella pyrenoidosa. I. Oxidation kinetics after a flash.

作者信息

Bouges-Bocquet B

出版信息

Biochim Biophys Acta. 1977 Nov 17;462(2):362-70. doi: 10.1016/0005-2728(77)90134-7.

DOI:10.1016/0005-2728(77)90134-7
PMID:588573
Abstract

P-700, plastocyanin and cytochrome f redox kinetics were measured after one flash, using dark-adapted Chlorella in the presence of hydroxylamine and 3(3,4-dichlorophenyl)-1,1-dimethylurea. Plastocyanin becomes increasingly oxidized with a half-time of 70 microseconds, then undergoes reduction with a half-time of 7ms. Cytochrome f oxidation has a sigmoidal time-course and a half-time of 100 microseconds. Its redution exhibits a half-time of 4 ms. These results are interpreted in a linear scheme: (formula: see text). An equilibrium constant of 2 between cytochrome f and plastocyanin (PC), which contrasts with the large equilibrium constant between PC and P-700 is computed. The presence of cytochrome b6 in a cyclic path around Photosystem I is confirmed under these conditions.

摘要

在存在羟胺和3-(3,4-二氯苯基)-1,1-二甲基脲的情况下,使用暗适应的小球藻,在一次闪光后测量P-700、质体蓝素和细胞色素f的氧化还原动力学。质体蓝素以70微秒的半衰期逐渐被氧化,然后以7毫秒的半衰期进行还原。细胞色素f的氧化具有S形时间进程,半衰期为100微秒。其还原表现出4毫秒的半衰期。这些结果用线性模式解释:(公式:见原文)。计算出细胞色素f和质体蓝素(PC)之间的平衡常数为2,这与PC和P-700之间的大平衡常数形成对比。在这些条件下证实了细胞色素b6在围绕光系统I的循环途径中的存在。

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Cytochrome f and plastocyanin kinetics in Chlorella pyrenoidosa. I. Oxidation kinetics after a flash.小球藻中细胞色素f和质体蓝素的动力学。I. 闪光后的氧化动力学。
Biochim Biophys Acta. 1977 Nov 17;462(2):362-70. doi: 10.1016/0005-2728(77)90134-7.
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J Biochem. 1984 Aug;96(2):385-93. doi: 10.1093/oxfordjournals.jbchem.a134849.

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Photosynth Res. 1991 Jul;29(1):45-54. doi: 10.1007/BF00035205.
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Electrochromic absorbance changes in the chlorophyll-c-containing alga Pleurochloris meiringensis (Xanthophyceae).
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