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来自光系统II的质子演化。化学计量与机理考量。

Proton evolution from photosystem II. Stoichiometry and mechanistic considerations.

作者信息

Fowler C F

出版信息

Biochim Biophys Acta. 1977 Nov 17;462(2):414-21. doi: 10.1016/0005-2728(77)90139-6.

DOI:10.1016/0005-2728(77)90139-6
PMID:588576
Abstract
  1. In a sequence of flashes given to dark-adapted chloroplasts, the flash yield of proton release oscillates with a period of 4, which is similar but not identical to the oscillation of the O2 flash yield. 2. Using the proton release associated with ferricyanide reduction as a calibration, we computed that two protons are released in the terminal O2-liberating reaction; the other two protons are released in precursor conversion steps. 3. Analysis of the effect of preflashes on the oscillation pattern showed that the S1 leads to S2 transition releases no proton, the S0 leads to S1 transition somewhat less than one (0.75), and the S2 leads to S3 transition somewhat more than one (1.25). 4. The precision of the data was sufficient to exclude the possibility that in the four-step water oxidation, proton release follows a simple 1, 0, 1, 2 pattern. A possible model to interpret the observed flash yield patterns is discussed.
摘要
  1. 在给予暗适应叶绿体的一系列闪光中,质子释放的闪光产额以4的周期振荡,这与O2闪光产额的振荡相似但不完全相同。2. 以与铁氰化物还原相关的质子释放作为校准,我们计算出在最终的O2释放反应中有两个质子被释放;另外两个质子在前体转化步骤中被释放。3. 对预闪光对振荡模式影响的分析表明,S1到S2的转变不释放质子,S0到S1的转变略少于一个(0.75),S2到S3的转变略多于一个(1.25)。4. 数据的精度足以排除在四步水氧化过程中质子释放遵循简单的1、0、1、2模式的可能性。讨论了一个可能解释观察到的闪光产额模式的模型。

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引用本文的文献

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Gernot Renger (1937-2013): his life, Max-Volmer Laboratory, and photosynthesis research.格诺特·伦格(1937 - 2013):他的生平、马克斯 - 沃尔默实验室与光合作用研究。
Photosynth Res. 2016 Aug;129(2):109-27. doi: 10.1007/s11120-016-0280-8. Epub 2016 Jun 16.
2
The mechanism of photosynthetic water oxidation.光合作用水氧化的机制。
Photosynth Res. 1985 Mar;6(1):33-55. doi: 10.1007/BF00029045.
3
On the action of hydroxylamine, hydrazine and their derivatives on the water-oxidizing complex.羟胺、肼及其衍生物对水氧化复合物的作用。
Photosynth Res. 1986 Jan;9(1-2):197-210. doi: 10.1007/BF00029744.
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Reaction sequences from light absorption to the cleavage of water in photosynthesis : Routes, rates and intermediates.光合作用中从光吸收到水裂解的反应序列:途径、速率和中间体。
Photosynth Res. 1986 Jan;10(3):453-71. doi: 10.1007/BF00118312.
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pH dependent stabilization of S2Q A (-) and S 2Q B (-) charge pairs studied by thermoluminescence.通过热致发光研究 S2Q A(-)和 S2Q B(-)电荷对的 pH 值依赖性稳定。
Photosynth Res. 1986 Jan;10(3):431-6. doi: 10.1007/BF00118309.
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Electron transfer through photosystem II acceptors: Interaction with anions.电子通过光系统 II 受体的转移:与阴离子的相互作用。
Photosynth Res. 1986 Jan;10(3):365-79. doi: 10.1007/BF00118302.
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Chloride binding proteins: mechanistic implications for the oxygen-evolving complex of Photosystem II.氯离子结合蛋白:对光系统 II 放氧复合物的机制影响。
Photosynth Res. 1990 Jan;23(1):1-27. doi: 10.1007/BF00030059.
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