放射免疫测定法与淋巴细胞毒性抑制技术在测定β2微球蛋白方面的比较。
Comparison of radio-immunoassay and lymphocytotoxicity inhibition techniques for the determination of beta2 microglobulin.
作者信息
Vincent C, Revillard J P
出版信息
J Immunol Methods. 1976 Mar;10(2-3):253-9. doi: 10.1016/0022-1759(76)90175-7.
Rabbit anti-human beta2 microglobulin antisera can lyse human lymphocytes in the presence of rabbit complement. Inhibition of the lymphocytotoxic reaction by highly purified beta2m was applied to the measurement of beta2m concentration in biological fluids. Parallel determinations were also performed using a radioimmunoassay. Lymphocytotoxicity inhibition is simple and more sensitive than radial immunodiffusion but less sensitive than the radioimmunoassay. beta2m was measured by these two techniques in serum and urine from normal individuals, uremic or transplanted patients.
兔抗人β2微球蛋白抗血清在兔补体存在的情况下能溶解人淋巴细胞。利用高度纯化的β2微球蛋白对淋巴细胞毒性反应的抑制作用来测定生物体液中的β2微球蛋白浓度。同时也采用放射免疫测定法进行平行测定。淋巴细胞毒性抑制法操作简单，比单向免疫扩散法更灵敏，但不如放射免疫测定法灵敏。运用这两种技术对正常个体、尿毒症患者或移植患者的血清和尿液中的β2微球蛋白进行了测定。
Zotero 插件