Bernard A M, Vyskocil A, Lauwerys R R
Clin Chem. 1981 Jun;27(6):832-7.
This highly sensitive method for determination of beta 2-microglobulin (beta 2-m) in human urine or serum is based on direct agglutination by beta 2-m of latex particles on which an antibody against beta 2-m is adsorbed. The agglutination is quantified by counting the remaining unagglutinated particles, or by turbidimetry. A novel aspect of this method is the capability to prevent nonspecific agglutination of the antibody-coated particles by diluting them with an albumin solution of well-defined characteristics (pH, freshness, concentration) just before the assay. The assayable concentration range is 1--32 micrograms/L, the detection limit 0.5 micrograms/L. Within-assay CV, based on 10 determinations of beta 2-m in urine and serum at two different dilutions, ranged from 4.6 to 8.7%. Between-assay CV, calculated from 10 determinations of beta 2-m in urine and serum, was 10 and 8.4%, respectively. Analytical recovery of beta 2-m in urine averaged 97% and in serum 104% (n = 10). No component of urine or serum interfered. Coefficients of correlation for beta 2-m in urine or serum as measured by radioimmunoassay and latex immunoassay were 0.97 and 0.93, respectively. Concentrations of beta 2-m in serum and urine from 33 healthy men (ages 20 to 67 years) averaged 1.5 mg/l and 54 micrograms/g of creatine, respectively.
这种用于测定人尿或血清中β2-微球蛋白（β2-m）的高灵敏度方法基于β2-m对吸附有抗β2-m抗体的乳胶颗粒的直接凝集作用。通过计数剩余的未凝集颗粒或比浊法对凝集进行定量。该方法的一个新特点是在测定前用具有明确特性（pH、新鲜度、浓度）的白蛋白溶液稀释抗体包被颗粒，从而防止其非特异性凝集。可测定浓度范围为1 - 32微克/升，检测限为0.5微克/升。基于在两种不同稀释度下对尿和血清中β2-m进行10次测定，批内变异系数在4.6%至8.7%之间。由对尿和血清中β2-m进行10次测定计算得出的批间变异系数分别为10%和8.4%。尿中β2-m的分析回收率平均为97%，血清中为104%（n = 10）。尿或血清的成分均无干扰。放射免疫测定法和乳胶免疫测定法测定的尿或血清中β2-m的相关系数分别为0.97和0.93。33名健康男性（年龄20至67岁）血清和尿中β2-m的浓度分别平均为1.5毫克/升和54微克/克肌酐。
