作为2,4-二硝基苯腙的缩醛磷脂醛的定量微量测定与分离
Quantitative micro determination and isolation of plasmalogen aldehydes as 2,4-dinitrophenylhydrazones.
作者信息
Katz I, Keeney M
出版信息
J Lipid Res. 1966 Jan;7(1):170-4.
PMID:5900215
Abstract
A micro spectrophotometric procedure for the quantitative determination of plasmalogen aldehydes is described which utilizes simultaneous methanolysis and formation of 2,4-dinitrophenylhydrazones. After isolation of the hydrazones by thin-layer chromatography, the aldehydes can be regenerated, reduced, acetylated, and then analyzed by gas-liquid chromatography. Identification of the plasmalogen aldehydes obtained from rumen holotrich protozoa is described.
摘要
本文描述了一种用于定量测定缩醛磷脂醛的微量分光光度法，该方法利用同时进行的甲醇解和2,4-二硝基苯腙的形成。通过薄层色谱法分离腙后，醛可以再生、还原、乙酰化，然后通过气液色谱法进行分析。文中还描述了从瘤胃全毛原生动物中获得的缩醛磷脂醛的鉴定。
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