使用2,4-二硝基苯肼测定蛋白质和肽中反应性羰基的微量方法。
Micro method for determination of reactive carbonyl groups in proteins and peptides, using 2,4-dinitrophenylhydrazine.
作者信息
Fields R, Dixon H B
出版信息
Biochem J. 1971 Feb;121(4):587-9. doi: 10.1042/bj1210587.
Abstract
A method is described for determining carbonyl groups that is especially suitable for use with proteins and peptides. It involves the determination of the extinction at 370nm of a sample solution after adding 2,4-dinitrophenylhydrazine. The reaction of 2,4-dinitrophenylhydrazine with pyruvoylglycine and with transaminated ribonuclease T(1) is presented; the isolation of protein hydrazones is discussed.
摘要
描述了一种测定羰基的方法，该方法特别适用于蛋白质和肽。它涉及在加入2,4-二硝基苯肼后测定样品溶液在370nm处的吸光度。介绍了2,4-二硝基苯肼与丙酮酰甘氨酸以及与转氨核糖核酸酶T(1)的反应；讨论了蛋白质腙的分离。
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