Linkie D M
Endocrinology. 1977 Dec;101(6):1862-70. doi: 10.1210/endo-101-6-1862.
The time-related uptake, retention and intracellular distribution of estrogen by different target tissues of the intact immature female rat were studied. The cytoplasmic compartment of the uterus, hypothalamus and pituitary was shown to have a primary hormone receptor complex sedimenting as 8S by sucrose density gradient analysis. Nuclear extracts from all three target tissues contained two forms of estrogen receptor complex; a 5--6S form and a more slowly sedimenting 4S species. The heavier nuclear form accumulated subsequent to the 4S complex in a time-dependent manner which differed according to target tissue. The observed intranuclear differences in properties of the estrogen receptor system may be related to the physiologic responses which characterize dissimilar estrogen target tissues. The tissue content of such serum estrogen-binding proteins as alpha1-fetoprotein and albumin were also determined. The observed levels were evaluated as possible factors in tissue estrogen retention.
研究了完整未成熟雌性大鼠不同靶组织中雌激素与时间相关的摄取、保留及细胞内分布情况。通过蔗糖密度梯度分析表明，子宫、下丘脑和垂体的细胞质部分含有一种主要的激素受体复合物，沉降系数为8S。来自所有这三个靶组织的核提取物含有两种形式的雌激素受体复合物：一种5 - 6S的形式和一种沉降更慢的4S种类。较重的核形式在4S复合物之后以时间依赖性方式积累，且根据靶组织的不同而有所差异。雌激素受体系统在核内观察到的性质差异可能与不同雌激素靶组织所特有的生理反应有关。还测定了诸如α1-胎蛋白和白蛋白等血清雌激素结合蛋白的组织含量。将观察到的水平评估为组织雌激素保留的可能因素。
