Martin P M, Sheridan P J
Experientia. 1980 May 15;36(5):620-2. doi: 10.1007/BF01965839.
The intracellular distribution of unbound estrogen receptor was estimated using nonaqueous and aqueous isolation of nuclei and autoradiography. The estimated amount of nuclear receptor varied greatly with the procedure used and thus caution is urged in approximating the in vivo intracellular distribution of receptors.
采用细胞核的非水性和水性分离及放射自显影法估算未结合雌激素受体的细胞内分布。所估算的核受体量因所用方法的不同而有很大差异,因此在估算受体的体内细胞内分布时需谨慎。