黑麦草（多年生黑麦草）提取物对4-氨基嘧啶核苷的脱氨作用。
Deamination of 4-aminopyrimidine nucleosides by extracts of rye grass (Lolium perenne).
作者信息
Frisch D M, Charles M A
出版信息
Plant Physiol. 1966 Mar;41(3):475-8. doi: 10.1104/pp.41.3.475.
Abstract
A deaminase specific for 4-aminopyrimidine nucleosides has been found in rye grass. Lolium perenne. The pH optimum, temperature stability, Km values and specificity was determined.A new and more accurate method for following the course of deamination is reported. This method depends on the shift in maximum absorbance wavelength of the mixture containing the 4-aminopyrimidine nucleoside and its deaminated product.
摘要
在黑麦草（多年生黑麦草）中发现了一种对4-氨基嘧啶核苷具有特异性的脱氨酶。测定了其最适pH值、温度稳定性、米氏常数（Km值）和特异性。报道了一种跟踪脱氨过程的新的更准确的方法。该方法依赖于含有4-氨基嘧啶核苷及其脱氨产物的混合物最大吸收波长的变化。
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