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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The enzymatic deamination of 6-aminopyrimidine deoxyribonucleotides. I. The enzymatic deamination of deoxycytidine 5'-phosphate and of 5-methyldeoxycytidine 5-methyldeoxycytidine 5'-phosphate.
J Biol Chem. 1960 Mar;235:706-13.
3
The enzymatic deamination of 5'-deoxycytidylic acid and of 5-methyl-5'-deoxycytidylic acid in the developing sea urchin embryo.发育中的海胆胚胎中5'-脱氧胞苷酸和5-甲基-5'-脱氧胞苷酸的酶促脱氨作用。
Exp Cell Res. 1959 Oct;18:333-46. doi: 10.1016/0014-4827(59)90012-6.
4
Studies on the metabolism of 5-iodo-2'-deoxycytidine in vitro. Purification of nucleoside deaminase from mouse kidney.5-碘-2'-脱氧胞苷的体外代谢研究。从小鼠肾脏中纯化核苷脱氨酶。
J Biol Chem. 1963 May;238:1772-6.
5
Deamination of deoxycytidine and 5-methyldeoxycytidine in developing anthers of Lilium longiflorum (var. Croft).麝香百合（克罗夫特变种）发育中的花药中脱氧胞苷和5-甲基脱氧胞苷的脱氨基作用。
J Biophys Biochem Cytol. 1961 Feb;9(2):279-84. doi: 10.1083/jcb.9.2.279.
6
Enzymatic deamination of deoxyadenylic and adenylic acids by normal and cancerous rat liver tissues.正常和癌变大鼠肝脏组织对脱氧腺苷酸和腺苷酸的酶促脱氨基作用。
J Natl Cancer Inst. 1961 May;26:1059-73.

黑麦草（多年生黑麦草）提取物对4-氨基嘧啶核苷的脱氨作用。

Deamination of 4-aminopyrimidine nucleosides by extracts of rye grass (Lolium perenne).

作者信息

Frisch D M, Charles M A

出版信息

Plant Physiol. 1966 Mar;41(3):475-8. doi: 10.1104/pp.41.3.475.

DOI:10.1104/pp.41.3.475
PMID:5906378
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1086367/
Abstract

A deaminase specific for 4-aminopyrimidine nucleosides has been found in rye grass. Lolium perenne. The pH optimum, temperature stability, Km values and specificity was determined.A new and more accurate method for following the course of deamination is reported. This method depends on the shift in maximum absorbance wavelength of the mixture containing the 4-aminopyrimidine nucleoside and its deaminated product.

摘要

在黑麦草（多年生黑麦草）中发现了一种对4-氨基嘧啶核苷具有特异性的脱氨酶。测定了其最适pH值、温度稳定性、米氏常数（Km值）和特异性。报道了一种跟踪脱氨过程的新的更准确的方法。该方法依赖于含有4-氨基嘧啶核苷及其脱氨产物的混合物最大吸收波长的变化。