酵母和一些哺乳动物物种中的线粒体DNA。

Mitochondrial DNA in yeast and some mammalian species.

作者信息

Corneo G, Moore C, Sanadi D R, Grossman L I, Marmur J

出版信息

Science. 1966 Feb 11;151(3711):687-9. doi: 10.1126/science.151.3711.687.

DOI:10.1126/science.151.3711.687

Abstract

Yeast DNA, in a cesium chloride density gradient, shows a minor or satellite band with a density lower than that of the main nuclear component. The DNA isolated from purified mitochondria of yeasts corresponds in density to this satellite band. In solution, this DNA more easily undergoes renaturation as compared to DNA from cell nuclei. The ease of this renaturation is presumably due to a homogeneity greater than that of nuclear DNA. Mitochondrial DNA isolated from several mammalian species has the same or higher density than nuclear DNA, but differs in its ready renaturability.

摘要

在氯化铯密度梯度中，酵母DNA呈现出一条密度低于主要核成分的次要带或卫星带。从酵母纯化线粒体中分离出的DNA在密度上与这条卫星带相对应。在溶液中，与细胞核DNA相比，这种DNA更容易复性。这种复性的容易程度大概是由于其比核DNA具有更高的均一性。从几种哺乳动物物种中分离出的线粒体DNA具有与核DNA相同或更高的密度，但其复性的难易程度有所不同。