酵母中的线粒体卫星和环状DNA细丝。

Mitochondrial-satellite and circular DNA filaments in yeast.

作者信息

Sinclair J H, Stevens B J, Sanghavi P, Rabinowitz M

出版信息

Science. 1967 Jun 2;156(3779):1234-7. doi: 10.1126/science.156.3779.1234.

DOI:10.1126/science.156.3779.1234

Abstract

Mitochondrial DNA of Saccharomyces cerevisiae contains a satellite DNA (density, 1.682) that appears to exist as open-ended filaments at least 5 microns long. DNA from intact cells contains circular filaments whose lengths vary from 0.5 to 7 microns, with a great majority at 1.95 microns. The circular DNA has a density similar to that of the major nuclear peak (1.697). When heat-denatured mitochondrial-satellite DNA is renatured, it cross-links to form a molecule that is larger than the native molecule. The formation of cross-links results in hypersharpening of the density profiles in cesium chloride and also leads to failure to pass Millipore filter paper.

摘要

酿酒酵母的线粒体DNA含有一种卫星DNA（密度为1.682），它似乎以至少5微米长的无端细丝形式存在。完整细胞中的DNA含有长度从0.5到7微米不等的环形细丝，绝大多数为1.95微米。环形DNA的密度与主要核峰的密度相似（1.697）。当热变性的线粒体卫星DNA复性时，它会交联形成一个比天然分子更大的分子。交联的形成导致氯化铯密度分布图的超锐化，并且还导致无法通过微孔滤纸。