Jamasbi R J, Nettesheim P
Int J Cancer. 1977 Dec 15;20(6):817-23. doi: 10.1002/ijc.2910200602.
The chemically induced, non-immunogenic lung squamous-cell carcinoma (MSC-10) propagated in vitro gradually loses tumorigenicity in immunocompetent hosts with increasing in vitro passage. This was found to be related to an increase in antigenicity, since immunosuppressed hosts (thymectomy plus 600 rads whole body X-irradiation) supported the growth of tumor cells, whereas immunocompetent recipients did not. The antigens involved in rejection are not heterologous serum proteins present in culture media since the cell line grown in isologous serum is also rejected. Immunization with the in vitro tumor line partially protected against the parental in vivo line, therefore the antigens involved must be present on both tumor lines. Inoculation of the cultured cell line into normal or immunosuppressed hosts produced tumors with the same histological characteristics as those of the in vivo tumor line. We concluded that by in vitro culture the weakly antigenic carcinoma becomes more immunogenic and thereby capable of inducing transplantation resistance. The cultured tumor cells retain their antigenic specificity and histologic characteristics while increasing their antigenic potency.
在体外传代培养过程中，化学诱导产生的、无免疫原性的肺鳞状细胞癌（MSC-10）在免疫活性宿主中会逐渐丧失致瘤性，且随着体外传代次数的增加，这种丧失致瘤性的现象愈发明显。研究发现，这一现象与抗原性的增加有关，因为免疫抑制宿主（胸腺切除加600拉德全身X射线照射）能够支持肿瘤细胞的生长，而免疫活性宿主则不能。参与排斥反应的抗原并非培养基中存在的异源血清蛋白，因为在同源血清中生长的细胞系同样会被排斥。用体外肿瘤细胞系进行免疫可部分保护机体免受亲代体内肿瘤细胞系的侵害，因此相关抗原必定同时存在于两种肿瘤细胞系中。将培养的细胞系接种到正常或免疫抑制宿主中，所产生肿瘤的组织学特征与体内肿瘤细胞系产生的肿瘤相同。我们得出结论，通过体外培养，弱抗原性癌变得更具免疫原性，从而能够诱导移植抗性。培养的肿瘤细胞在增加抗原效力的同时，保留了其抗原特异性和组织学特征。
