酵母需氧生长周期中干重、蛋白质、脱氧核糖核酸、核糖核酸以及储备和结构碳水化合物的变化。

Changes in dry weight, protein, deoxyribonucleic acid, ribonucleic acid and reserve and structural carbohydrate during the aerobic growth cycle of yeast.

Polakis E S, Bartley W

Biochem J. 1966 Mar;98(3):883-7. doi: 10.1042/bj0980883.

Abstract

Changes in dry weight, DNA, RNA, protein and reserve and structural carbohydrate were measured during the aerobic growth of yeast on 0.9% glucose in an aerobic synthetic medium. 2. After glucose had been consumed and during the growth of yeast on ethanol and acetate, the rate of formation of DNA remained about the same but the rate of increase of dry weight was greatly diminished. 3. During the second stage of growth the ratios dry weight/DNA, protein/DNA, RNA/DNA and carbohydrate/DNA decreased to about 30% of the corresponding values during the first stage of growth. 4. A higher fraction of the dry weight of the yeast cells could be accounted for by the reserve carbohydrate content of the cells during the second stage of growth. 5. By the end of the first stage of growth an increase in the reserve carbohydrate content of the cells was observed. Part of this reserve carbohydrate was consumed by the cells in the beginning of the second stage of growth. The possibility of adaptation of cells at the expense of their reserves is discussed.

摘要

在有氧合成培养基中，于0.9%葡萄糖上进行酵母的有氧生长期间，测定了干重、DNA、RNA、蛋白质以及储备和结构碳水化合物的变化。2. 葡萄糖被消耗后，以及在酵母于乙醇和乙酸盐上生长期间，DNA的形成速率保持大致相同，但干重的增加速率大幅降低。3. 在生长的第二阶段，干重/DNA、蛋白质/DNA、RNA/DNA和碳水化合物/DNA的比率降至生长第一阶段相应值的约30%。4. 在生长的第二阶段，酵母细胞干重的更高比例可由细胞的储备碳水化合物含量来解释。5. 到生长第一阶段结束时，观察到细胞的储备碳水化合物含量增加。在生长第二阶段开始时，细胞消耗了部分这种储备碳水化合物。讨论了细胞以其储备为代价进行适应的可能性。