Hartwell L H, McLaughlin C S
J Bacteriol. 1968 Nov;96(5):1664-71. doi: 10.1128/jb.96.5.1664-1671.1968.
Certain temperature-sensitive (ts(-)) mutants of yeast which cannot be corrected by nutritional supplementation exhibited a rapid cessation of protein synthesis after a shift to the restrictive temperature. Genetic and biochemical tests permitted a division of these mutants into four classes. This division was based upon genetic complementation patterns among the mutants and an investigation of glucose incorporation into macromolecules and polyribosome content in the mutants after a shift to the restrictive temperature. A study of these parameters in the parent strain (ts(+)) in the presence of certain well-characterized inhibitors allowed a tentative identification of the biochemical defects in each of the four classes. The properties of the mutants in class IA were consistent with the hypothesis that they result from a defect in the initiation of polypeptide chains or in ribonucleic acid synthesis; mutants in class IB from a defect in the elongation of polypeptide chains; mutants in class IIA from a defect in energy metabolism; and mutants in class IIB from a lesion affecting membrane function.
某些无法通过营养补充来纠正的酵母温度敏感（ts(-)）突变体在转移到限制温度后，蛋白质合成迅速停止。遗传和生化测试允许将这些突变体分为四类。这种分类基于突变体之间的遗传互补模式，以及对转移到限制温度后突变体中葡萄糖掺入大分子和多聚核糖体含量的研究。在存在某些特征明确的抑制剂的情况下，对亲本菌株（ts(+)）中的这些参数进行研究，初步确定了四类突变体中每一类的生化缺陷。IA类突变体的特性与它们是由多肽链起始或核糖核酸合成缺陷导致的假设一致；IB类突变体是由多肽链延伸缺陷导致；IIA类突变体是由能量代谢缺陷导致；IIB类突变体是由影响膜功能的损伤导致。
