猫小脑神经元的乙酰胆碱敏感性

Acetylcholine sensitivity of cerebellar neurones in the cat.

作者信息

Crawford J M, Curtis D R, Voorhoeve P E, Wilson V J

出版信息

J Physiol. 1966 Sep;186(1):139-65. doi: 10.1113/jphysiol.1966.sp008025.

DOI:10.1113/jphysiol.1966.sp008025

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1395899/

Abstract

Cholinomimetics, acetylcholine antagonists and some other compounds of pharmacological interest were administered electrophoretically near neurones within the vermal cerebellar cortex of anaesthetized (pentobarbitone) and unanaesthetized (cerveau isolé) cats.2. The neurones were identified by position within the cortex, spontaneous activity, and the responses to afferent and antidromic stimulation.3. Purkinje cells, but neither granule nor basket cells, were excited by cholinomimetics, and the acetylcholine receptors had muscarinic properties. Excitation was often preceded by depression of the spontaneous firing.4. Intravenously administered atropine and dihydro-beta-erythroidine did not depress the synaptic excitation of cerebellar neurones evoked by impulses in mossy, climbing or parallel fibres.5. Acetylcholine is thus unlikely to be an excitatory transmitter within the feline cerebellum, particularly at mossy fibre-granule cell synapses, despite the presence of relatively high levels of acetylcholinesterase within mossy fibre terminals.

摘要

在麻醉（戊巴比妥）和未麻醉（孤立脑）的猫的小脑蚓部皮质内，通过电泳法在神经元附近给予拟胆碱药、乙酰胆碱拮抗剂及其他一些具有药理学意义的化合物。
根据皮质内的位置、自发活动以及对传入和逆向刺激的反应来识别神经元。
拟胆碱药可兴奋浦肯野细胞，但对颗粒细胞和篮状细胞无此作用，且乙酰胆碱受体具有毒蕈碱样特性。兴奋之前常伴有自发放电的抑制。
静脉注射阿托品和二氢-β-刺桐定不会抑制由苔藓纤维、攀缘纤维或平行纤维中的冲动所诱发的小脑神经元的突触兴奋。
因此，尽管苔藓纤维终末内存在相对高水平的乙酰胆碱酯酶，但乙酰胆碱不太可能是猫小脑内的兴奋性递质，尤其是在苔藓纤维-颗粒细胞突触处。