采用酶联免疫吸附测定法检测急性弓形虫感染期间的循环抗原。
Detection of circulating antigen during acute infections with Toxoplasma gondii by enzyme-linked immunosorbent assay.
作者信息
van Knapen F, Panggabean S O
出版信息
J Clin Microbiol. 1977 Dec;6(6):545-7. doi: 10.1128/jcm.6.6.545-547.1977.
Abstract
The serodiagnosis of toxoplasmosis is usually based on demonstration of antibodies by means of different serological methods. However, for confirmation of active toxoplasmosis, examination of paired sera is still needed. In this study a description is given of a sensitive enzyme method (enzyme-linked immunosorbent assay) with which it is possible to detect circulating antigens during the acute stage of mouse and human toxoplasmosis. Preliminary investigations of human sera suggested that circulating antigens are only found during a short period (active phase) of the toxoplasma infection and that they can be found in cases of fresh infections as well as during reinfections. The possible clinical relevance is discussed.
摘要
弓形虫病的血清学诊断通常基于通过不同血清学方法证明抗体的存在。然而，为了确诊活动性弓形虫病，仍需要检测双份血清。在本研究中，描述了一种敏感的酶法（酶联免疫吸附测定），用该方法可以在小鼠和人类弓形虫病的急性期检测循环抗原。对人类血清的初步研究表明，循环抗原仅在弓形虫感染的短时期（活动期）内出现，并且在新感染病例以及再感染期间均可发现。文中还讨论了其可能的临床意义。
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