酶联免疫吸附测定（ELISA）及其他血清学检测用于诊断弓形虫病的评估。
Evaluation of the enzyme-linked immunosorbent assay (ELISA) and other serological tests for the diagnosis of toxoplasmosis.
作者信息
Carlier Y, Bout D, Dessaint J P, Capron A, Van Knapen F, Ruitenberg E J, Bergquist R, Huldt G
出版信息
Bull World Health Organ. 1980;58(1):99-105.
The enzyme-linked immunosorbent assay (ELISA) was evaluated in human toxoplasmosis in three laboratories using their own procedures. The same batch of serum samples was investigated in the three laboratories. ELISA results were compared by statistical analysis both with one another and with those of the dye test (DT), immunofluorescence (IF), complement fixation test (CFT), and indirect haemagglutination (IHA).Highly significant correlations were obtained between the three laboratories with ELISA using two different antigens and enzyme conjugates. The correlations between ELISA and the other serological tests showed the following sequence: CFT>IF>IHA>DT. Highly significant correlations were obtained between ELISA using anti-gamma-chain and anti-total immunoglobulin conjugates. The agreement in discrimination between sera with low and high antibody levels was good for all the different ELISA techniques but discrimination between positive and negative sera depended rather on the ELISA procedure used.
三个实验室采用各自的方法对酶联免疫吸附测定法（ELISA）在人类弓形虫病中的应用进行了评估。在这三个实验室中对同一批血清样本进行了检测。通过统计分析对ELISA结果相互之间以及与染色试验（DT）、免疫荧光法（IF）、补体结合试验（CFT）和间接血凝试验（IHA）的结果进行了比较。使用两种不同抗原和酶结合物的ELISA在三个实验室之间获得了高度显著的相关性。ELISA与其他血清学检测之间的相关性显示出以下顺序：CFT>IF>IHA>DT。使用抗γ链和抗总免疫球蛋白结合物的ELISA之间获得了高度显著的相关性。对于所有不同的ELISA技术，在区分低抗体水平和高抗体水平血清方面的一致性良好，但区分阳性和阴性血清则更多地取决于所使用的ELISA程序。
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