Lindenschmidt E G
Eur J Clin Microbiol. 1985 Oct;4(5):488-92. doi: 10.1007/BF02014430.
Sixty-two sera from 51 patients with lymphadenopathy presumed to be due to acute-phase toxoplasmosis were tested for specific IgM class antibodies by both the immunofluorescence antibody to Toxoplasma gondii in sera were first dissociated in 3 M NaSCN. Antigen attached to the solid phase was detected with enzyme-coupled IgG antibody to Toxoplasma antibody to Toxoplasma gondii in sera were first dissociated in 3 M NaSCN. Antigen attached to the solid phase was detected with enzyme-coupled IgG antibody to Toxoplasma gondii. Neither hepatitis B surface antigen nor antigen of Mycoplasma pneumoniae, rubella, cytomegalovirus or herpes simplex virus interfered with this ELISA. Soluble antigen was detected in 13 (30%) of 42 IgM-positive acute-phase toxoplasmosis patients and in only one of 20 sera cleared of IgM. None of an additional 44 IgM-negative patients with low IgG titres had a positive result in the antigen ELISA. Follow-up studies in four acute-phase toxoplasmosis patients showed that the soluble antigen cleared in all cases before the specific IgM antibodies. Simultaneous detection of IgM antibodies to Toxoplasma gondii and soluble antigen would thus seem to indicate an early stage of the infection.
对51例被认为因急性期弓形虫病导致淋巴结病患者的62份血清进行检测，通过免疫荧光法检测针对刚地弓形虫的特异性IgM类抗体，血清中的弓形虫抗体首先在3M硫氰酸钠中解离。固相上附着的抗原用酶联抗刚地弓形虫IgG抗体进行检测。血清中的弓形虫抗体首先在3M硫氰酸钠中解离。固相上附着的抗原用酶联抗刚地弓形虫IgG抗体进行检测。乙肝表面抗原、肺炎支原体、风疹、巨细胞病毒或单纯疱疹病毒的抗原均不干扰该酶联免疫吸附测定（ELISA）。在42例IgM阳性急性期弓形虫病患者中，有13例（30%）检测到可溶性抗原，而在20份已清除IgM的血清中仅1例检测到。另外44例IgG滴度低的IgM阴性患者在抗原ELISA中均无阳性结果。对4例急性期弓形虫病患者的随访研究表明，在特异性IgM抗体出现之前，所有病例中的可溶性抗原均已清除。因此，同时检测抗刚地弓形虫IgM抗体和可溶性抗原似乎表明处于感染的早期阶段。
