Dixon J S, Li C H
Science. 1966 Nov 11;154(3750):785-6. doi: 10.1126/science.154.3750.785.
The reduced alkylated derivative of human pituitary growth hormone was prepared by reacting the native hormone in 8M urea solution, first with mercaptoethanol and then with iodoacetamide. On the basis of amino acid analyses, the product isolated after gel filtration on Sephadex G-100 is completely devoid of unreduced cystine but otherwise has a composition identical to that of the native hormone. When assayed in both the rat tibia test and the pigeon crop-sac assay,it appears that the full potency of the native hormone is retained in the reduced alkylated derivative.
人垂体生长激素的还原烷基化衍生物是通过将天然激素在8M尿素溶液中，先与巯基乙醇反应，然后与碘乙酰胺反应制备而成。基于氨基酸分析，在Sephadex G - 100上进行凝胶过滤后分离得到的产物完全不含未还原的胱氨酸，但在其他方面其组成与天然激素相同。当在大鼠胫骨试验和鸽嗉囊试验中进行测定时，似乎天然激素的全部效力在还原烷基化衍生物中得以保留。
