Reagan C R, Mills J B, Kostyo J L, Wilhelmi A E
Proc Natl Acad Sci U S A. 1975 May;72(5):1684-6. doi: 10.1073/pnas.72.5.1684.
Reduction and carbamidomethylation of the intrachain disulfide bridges of human growth hormone did not destroy its ability to stimulate weight gain or cartilage metabolism in hypophysectomized rats. The reduced and alkylated hormone also stimulated glucose oxidation in isolated adipose tissue of hypophysectomized rats when added in vitro. When the S-carbamidomethylated hormone was incubated overnight with human plasmin, approximately 95% of the starting material was completely digested, as judged by polyacrylamide gel electrophoresis. The plasmin digest retained the ability to stimulate weight gain, cartilage metabolism, and glucose oxidation. A fraction consisting of two major electrophoretic components was isolated from the digest by chromatography on tsephadex G-50. This fraction possessed the biological properties of the whole digest.
人生长激素链内二硫键的还原和氨甲酰甲基化并未破坏其刺激垂体切除大鼠体重增加或软骨代谢的能力。当在体外添加时，还原和烷基化的激素也刺激了垂体切除大鼠分离的脂肪组织中的葡萄糖氧化。当S-氨甲酰甲基化激素与人纤溶酶一起孵育过夜时，通过聚丙烯酰胺凝胶电泳判断，约95%的起始物质被完全消化。纤溶酶消化产物保留了刺激体重增加、软骨代谢和葡萄糖氧化的能力。通过在葡聚糖G-50上进行色谱分离，从消化产物中分离出一个由两个主要电泳成分组成的组分。该组分具有整个消化产物的生物学特性。
