人生长激素：通过激素两个片段的非共价相互作用恢复完全生物活性。
Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone.
作者信息
Li C H, Bewley T A
出版信息
Proc Natl Acad Sci U S A. 1976 May;73(5):1476-9. doi: 10.1073/pnas.73.5.1476.
Abstract
The NH2-terminal 134 amino-acid fragment of the reduced-carbamidomethylated human somatropin molecule is found to react noncovalently with the COOH-terminal 51 amino-acid fragment in solutions of pH 8.4 at 2 degrees to restore full biological activity as evidenced by the rat tibia and pigeon crop-sac assays. In addition, circular dichroism spectra of the recombinant show the conformation to be completely repaired in comparison with that of the native hormone.
摘要
还原氨甲酰甲基化人生长激素分子的氨基末端134个氨基酸片段在pH 8.4、2℃的溶液中与羧基末端51个氨基酸片段发生非共价反应，恢复了全部生物活性，大鼠胫骨和鸽嗉囊试验证明了这一点。此外，重组体的圆二色光谱显示，与天然激素相比，其构象已完全修复。
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本文引用的文献
1
Bioassay of hypophyseal growth hormone; the tibia test.垂体生长激素的生物测定；胫骨试验。
Endocrinology. 1949 Nov;45(5):455-63, illust. doi: 10.1210/endo-45-5-455.
2
MOLECULAR WEIGHT OF SHEEP PITUITARY INTERSTITIAL CELL-STIMULATING HORMONE.绵羊垂体促间质细胞激素的分子量
Nature. 1964 Apr 18;202:291-2. doi: 10.1038/202291b0.
3
Human pituitary growth hormone. VI. Modified procedure of isolation and NH2-terminal amino acid sequence.人垂体生长激素。VI. 改进的分离方法及氨基末端氨基酸序列
Arch Biochem Biophys. 1962 Sep;Suppl 1:327-32.
4
Bio-assay of prolactin. Analysis of the pigeon crop-sac response to local prolactin injection by an objective and quantitative method.催乳素的生物测定。用客观定量方法分析鸽子嗉囊对局部注射催乳素的反应。
Endocrinology. 1967 Apr;80(4):641-55. doi: 10.1210/endo-80-4-641.
5
Retention of the biological potency of human pituitary growth hormone after reduction and carbamidomethylation.还原和氨甲酰甲基化后人垂体生长激素生物活性的保留
Science. 1966 Nov 11;154(3750):785-6. doi: 10.1126/science.154.3750.785.
6
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Biochim Biophys Acta. 1968 Feb 19;154(2):420-2. doi: 10.1016/0005-2795(68)90116-5.
7
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8
Human pituitary growth hormone. Physicochemical investigations of the native and reduced-alkylated protein.人生长激素。天然及还原烷基化蛋白质的物理化学研究。
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10
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Arch Biochem Biophys. 1974 Oct;164(2):571-4. doi: 10.1016/0003-9861(74)90068-x.
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