人生长激素：通过激素两个片段的非共价相互作用恢复完全生物活性。
Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone.
作者信息
Li C H, Bewley T A
出版信息
Proc Natl Acad Sci U S A. 1976 May;73(5):1476-9. doi: 10.1073/pnas.73.5.1476.
Abstract
The NH2-terminal 134 amino-acid fragment of the reduced-carbamidomethylated human somatropin molecule is found to react noncovalently with the COOH-terminal 51 amino-acid fragment in solutions of pH 8.4 at 2 degrees to restore full biological activity as evidenced by the rat tibia and pigeon crop-sac assays. In addition, circular dichroism spectra of the recombinant show the conformation to be completely repaired in comparison with that of the native hormone.
摘要
还原氨甲酰甲基化人生长激素分子的氨基末端134个氨基酸片段在pH 8.4、2℃的溶液中与羧基末端51个氨基酸片段发生非共价反应，恢复了全部生物活性，大鼠胫骨和鸽嗉囊试验证明了这一点。此外，重组体的圆二色光谱显示，与天然激素相比，其构象已完全修复。
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