Harkness D R
J Bacteriol. 1966 Sep;92(3):623-7. doi: 10.1128/jb.92.3.623-627.1966.
Harkness, Donald R. (University of Miami School of Medicine, Miami, Fla.). Bacterial growth on aminoalkylphosphonic acids. J. Bacteriol. 92:623-627. 1966.-Of 10 bacterial strains tested, 9 were found to be able to utilize the phosphorus of at least one of eight different aminoalkylphosphonic acids for growth, indicating that the ability to catabolize the carbon-phosphorus (C-P) bond is widespread among bacteria. Several organisms gave comparable growth rates as well as cell yields when an equimolar amount of either P(i) or 2-aminoethylphosphonic acid (2-AEP) was added to the medium. No compounds containing C-P bonds were detected in Escherichia coli B grown on 2-AEP(32)-orthophosphate. No degradation of phosphonates by cell-free extracts or suspensions of dried cells was demonstrated. The direct involvement of alkaline phosphatases in cleaving the C-P bond was excluded.
哈克尼斯，唐纳德·R.（迈阿密大学医学院，佛罗里达州迈阿密）。氨基烷基膦酸上的细菌生长。《细菌学杂志》92:623 - 627。1966年。在所测试的10种细菌菌株中，发现9种能够利用8种不同氨基烷基膦酸中至少一种的磷进行生长，这表明分解碳 - 磷（C - P）键的能力在细菌中广泛存在。当向培养基中添加等摩尔量的无机磷（P(i)）或2 - 氨基乙基膦酸（2 - AEP）时，几种生物体的生长速率和细胞产量相当。在以2 - AEP(32)-正磷酸盐为培养基生长的大肠杆菌B中未检测到含C - P键的化合物。未证明无细胞提取物或干燥细胞悬液对膦酸盐有降解作用。排除了碱性磷酸酶直接参与裂解C - P键的可能性。
