Brotherton J
J Reprod Fertil. 1977 Nov;51(2):383-91. doi: 10.1530/jrf.0.0510383.
Fifty-two (52) compounds were tested for spermicidal activity by titration against human spermatozoa. The gradual decrease in mean sperm size was measured against increasing concentration of spermicide and the end-point was taken as the point at which all the peripheral cytoplasm had been removed and only the sperm core of nucleus and tail fibres remained. There were 14 compounds that produced this total effect. All were detergents, of various types, and the effect was purely physical. The most potent compounds caused complete stripping at 0.5-50 pmol/cell and most are already used in spermicidal preparations. A further 11 compounds, including sodium hypochlorite and some phenols, caused partial stripping, while 4 compounds caused sperm swelling. The test was not suitable for assessment of metabolic cell poisons.
通过滴定法针对人类精子测试了52种化合物的杀精活性。随着杀精剂浓度的增加，测量精子平均大小的逐渐减小，终点定义为所有周边细胞质被去除，仅剩下精子的细胞核和尾部纤维核心的点。有14种化合物产生了这种完全效应。所有这些都是各种类型的洗涤剂，其效应纯粹是物理性的。最有效的化合物在0.5 - 50 pmol/细胞时导致完全剥离，并且大多数已用于杀精制剂中。另外11种化合物，包括次氯酸钠和一些酚类，导致部分剥离，而4种化合物导致精子肿胀。该测试不适用于评估代谢性细胞毒物。
