鸡细胞中的干扰素产生与蛋白质合成。
Interferon production and protein synthesis in chick cells.
作者信息
Friedman R M
出版信息
J Bacteriol. 1966 Mar;91(3):1224-9. doi: 10.1128/jb.91.3.1224-1229.1966.
Friedman, Robert M. (National Cancer Institute, Bethesda, Md.). Interferon production and protein synthesis in chick cells. J. Bacteriol. 91:1224-1229. 1966.-Overnight incubation of chick embryo fibroblasts (CEF) at 4 C before infection with live Semliki Forest virus (SFV) increased virus yields but decreased interferon production. The same findings were noted when CEF were incubated for 4 hr with p-fluorophenylalanine (FPA) before infection with live SFV or inactivated Chikungunya virus. In both systems incorporation of C(14)-leucine into protein appeared to be increased after pretreatment at 4 C or with FPA. Protein synthesis could be raised in CEF incubated in 0.5% serum after trypsinization by increasing the concentration of serum. CEF in 10% serum had higher rates of C(14)-leucine incorporation than did cells in 1.5% serum, but again the cells with the apparently high rate of incorporation produced less interferon. These findings may be related to the mechanism of cellular control over interferon production.
弗里德曼，罗伯特·M.（国立癌症研究所，马里兰州贝塞斯达）。鸡细胞中的干扰素产生与蛋白质合成。《细菌学杂志》91:1224 - 1229。1966年。——在用活的Semliki森林病毒（SFV）感染鸡胚成纤维细胞（CEF）之前，将其在4℃过夜培养，可提高病毒产量，但会降低干扰素产生。在用活的SFV或灭活的基孔肯雅病毒感染之前，若将CEF与对氟苯丙氨酸（FPA）一起孵育4小时，也会有相同的发现。在这两种系统中，经4℃预处理或用FPA处理后，C（14）-亮氨酸掺入蛋白质的量似乎都增加了。胰蛋白酶消化后，在0.5%血清中培养的CEF中，通过提高血清浓度可提高蛋白质合成。在10%血清中的CEF比在1.5%血清中的细胞有更高的C（14）-亮氨酸掺入率，但同样，掺入率明显较高的细胞产生的干扰素较少。这些发现可能与细胞对干扰素产生的控制机制有关。
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