Johnson A H, Stokes J L
J Bacteriol. 1966 Apr;91(4):1543-7. doi: 10.1128/jb.91.4.1543-1547.1966.
Johnson, A. H. (Washington State University, Pullman), and J. L. Stokes. Manganese oxidation by Sphaerotilus discophorus. J. Bacteriol. 91:1543-1547. 1966.-Cell suspensions of Sphaerotilus discophorus in 0.02 m potassium phosphate buffer (pH 7.2) readily oxidized MnSO(4) to dark-brown manganic oxide. The latter appeared to be MnO(2), since its color was the same as MnO(2) and, like the latter, it was readily soluble in HCl with the evolution of chlorine. The amount of O(2) consumed in the oxidation of Mn(++) was 63% or more of theoretical. The ability of the cells to oxidize Mn(++) was destroyed by heating the cells at 93 C for 5 min. Only cell suspensions prepared from cultures of S. discophorus grown with MnSO(4) were able to oxidize Mn(++). It was concluded that the oxidation of manganous ions by S. discophorus is catalyzed by an inducible enzyme(s).
约翰逊，A. H.（华盛顿州立大学，普尔曼）和J. L. 斯托克斯。盘状球衣菌对锰的氧化作用。《细菌学杂志》91:1543 - 1547。1966年。——在0.02m磷酸钾缓冲液（pH 7.2）中的盘状球衣菌细胞悬液能轻易地将硫酸锰氧化为深棕色的锰氧化物。后者似乎是二氧化锰，因为其颜色与二氧化锰相同，并且和二氧化锰一样，它能在盐酸中轻易溶解并释放出氯气。在氧化二价锰过程中消耗的氧气量为理论值的63%或更多。通过在93℃加热细胞5分钟，细胞氧化二价锰的能力被破坏。只有用含有硫酸锰培养的盘状球衣菌培养物制备的细胞悬液能够氧化二价锰。得出的结论是，盘状球衣菌对锰离子的氧化作用是由一种诱导酶催化的。
