Corstjens P L, de Vrind J P, Westbroek P, de Vrind-de Jong E W
Department of Biochemistry, Leiden University, The Netherlands.
Appl Environ Microbiol. 1992 Feb;58(2):450-4. doi: 10.1128/aem.58.2.450-454.1992.
An iron-oxidizing factor was identified in the spent culture medium of the iron- and manganese-oxidizing bacterial strain Leptothrix discophora SS-1. It appeared to be a protein, with an apparent molecular weight of approximately 150,000. Its activity could be demonstrated after fractionation of the spent medium by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A spontaneous mutant of L. discophora SS-1 was isolated which excreted neither manganese- nor iron-oxidizing activity, whereas excretion of other proteins seemed to be unaffected. Although the excretion of both metal-oxidizing factors was probably linked, the difference in other properties suggests that manganese and iron oxidation represent two different pathways. With a dot-blot assay, it was established that different bacterial species have different metal-oxidizing capacities. Whereas L. discophora oxidized both iron and manganese, Sphaerotilus natans oxidized only iron and two Pseudomonas spp. oxidized only manganese.
在铁和锰氧化细菌菌株盘状纤发菌（Leptothrix discophora）SS-1的废弃培养基中鉴定出一种铁氧化因子。它似乎是一种蛋白质，表观分子量约为150,000。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对废弃培养基进行分级分离后，可证明其活性。分离出盘状纤发菌SS-1的一个自发突变体，该突变体既不分泌锰氧化活性也不分泌铁氧化活性，而其他蛋白质的分泌似乎未受影响。尽管两种金属氧化因子的分泌可能相关，但其他特性的差异表明锰氧化和铁氧化代表两种不同的途径。通过斑点印迹分析确定，不同的细菌物种具有不同的金属氧化能力。盘状纤发菌能氧化铁和锰，浮游球衣菌仅能氧化铁，而两种假单胞菌属仅能氧化锰。
