Miguel S G
Lipids. 1977 Dec;12(12):1080-3. doi: 10.1007/BF02533338.
Slices of rat jejunum were incubated with [2(-14)C] pyruvate, [1(-14)C] acetate, or [3H]H2O to determine lipogenic activity. Under all conditions studied, pyruvate acted as a better precursor than acetate for fatty acid synthesis but not for the synthesis of sterol. Exogenous glucose significantly (P less than or equal to 0.05) increased the conversion of both pyruvate and acetate to fatty acids. By contrast fasting resulted in a decrease (p less than or equal to 0.05) in lipogenic activity. The highest levels of lipogenesis were observed when [3H]H2O + glucose at a concentration of 20 mM was used. From such experiments, the absolute rate of fatty acid synthesis in the tissue preparation was calculated: 734 +/- 54 nmoles acetyl units incorporated into fatty acids/g tissue/hr.
将大鼠空肠切片与[2(-14)C]丙酮酸、[1(-14)C]乙酸盐或[3H]H2O一起孵育，以测定脂肪生成活性。在所有研究条件下，丙酮酸作为脂肪酸合成的前体比乙酸盐更好，但对于固醇合成则不然。外源性葡萄糖显著（P≤0.05）增加了丙酮酸和乙酸盐向脂肪酸的转化。相比之下，禁食导致脂肪生成活性降低（p≤0.05）。当使用浓度为20 mM的[3H]H2O + 葡萄糖时，观察到最高水平的脂肪生成。通过此类实验，计算出组织制剂中脂肪酸合成的绝对速率：734±54纳摩尔乙酰单位掺入脂肪酸/克组织/小时。
