Celada F, Wigzell H
Immunology. 1966 Mar;10(3):231-43.
Antibody-forming cells were found in autochthonous spleen colonies, repopulating the spleens of mice which had been exposed to mid-lethal doses of radiation and inoculation of sheep red blood cells. This was true only for the secondary response, where a proportion of colonies contained haemolytic plaque-forming cells in variable numbers, ranging from 1/10 to 1/10 nucleated cells within a given spleen. The frequency distribution of antibody-forming cells in colonies suggests a clonal growth at an exponential rate, with a generation time of about 7 hours. It is therefore possible to cultivate and study single clones of competent cells in readily accessible, anatomically distinct regions of the mouse spleen. The present data do not discriminate between the derivation of the antibody-forming clones from the original colony-forming cells, or from a cell seeded-in from the spleen tissue outside the colony.
在自体脾集落中发现了抗体形成细胞，这些集落重新填充了接受中等致死剂量辐射并接种绵羊红细胞的小鼠的脾脏。这仅在二次反应中成立，在二次反应中，一定比例的集落含有数量不等的溶血空斑形成细胞，在给定脾脏内，其数量范围为每10个有核细胞中有1/10到1/100个。集落中抗体形成细胞的频率分布表明其以指数速率进行克隆生长，代时约为7小时。因此，有可能在小鼠脾脏易于接近、解剖学上不同的区域培养和研究单个的有功能细胞克隆。目前的数据无法区分抗体形成克隆是源自原始集落形成细胞，还是源自从集落外的脾脏组织中植入的细胞。
