哈特利豚鼠对牛胰岛素的二次抗体反应的若干方面；使用化学修饰的牛胰岛素来确定牛胰岛素的抗原决定簇。
Aspects of the secondary antibody response to ox insulin in the Hartley guinea-pig; the use of chemically modified ox insulin to delineate the antigenic determinants of ox insulin.
作者信息
Hollins P J, Himsworth R L
出版信息
Clin Exp Immunol. 1976 Mar;23(3):507-16.
The use of a quinea-pig model to study the immunogenicity of the insulin molecule is presented. The Hartley guinea-pig has been shown consistently to form antibody to ox insulin, when given in a water-in-oil emulsion containing pertussis vaccine as adjuvant. After log transformation of standardized antibody titres to iodo-ox insulin, a valid statistical comparison of the antibody response to different ox insulin preparations could be made. Antibody cross-reacting with ox insulin, but not iodo-ox insulin, was also detected. The quantity of one type of antibody was complementary to the other, an observation compatible with determinant competition having occurred during the immune response. From the results of cross-reactivity experiments using N-triacylated ox insulins and human insulin, it was shown that antibody cross-reacting with iodo-ox insulin had most probably been produced to a localized area of the molecule.
本文介绍了使用豚鼠模型研究胰岛素分子免疫原性的情况。当将哈特利豚鼠在含有百日咳疫苗作为佐剂的油包水乳液中给予牛胰岛素时，已证明其能持续产生针对牛胰岛素的抗体。在将标准化抗体滴度对碘代牛胰岛素进行对数转换后，可以对不同牛胰岛素制剂的抗体反应进行有效的统计学比较。还检测到了与牛胰岛素交叉反应但不与碘代牛胰岛素交叉反应的抗体。一种抗体的量与另一种抗体互补，这一观察结果与免疫反应期间发生的决定簇竞争相符。从使用N - 三酰化牛胰岛素和人胰岛素的交叉反应实验结果表明，与碘代牛胰岛素交叉反应的抗体很可能是针对该分子的一个局部区域产生的。
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