从产蛋母鸡子宫中纯化钙结合蛋白。

The purification of calcium-binding protein from the uterus of the laying hen.

作者信息

Fullmer C S, Brindak M E, Bar A, Wasserman R H

出版信息

Proc Soc Exp Biol Med. 1976 Jun;152(2):237-41. doi: 10.3181/00379727-152-39369.

Abstract

Calcium-binding proteins from the chick intestine and uterus of the laying hen (Gallus domesticus) were isolated and purified by identical methods. Proteins from each source were shown to exhibit identical electrophoretic mobility and were immunologically identical. Estimation of molecular size by gel filtration indicated a value of approximately 28,000 daltons for both the chick intestinal and hen uterine calcium-binding proteins. The amino acid compositions of chick intestinal and hen uterine calcium-binding proteins were essentially identical.

摘要

采用相同方法从产蛋母鸡（家鸡）的小肠和子宫中分离并纯化了钙结合蛋白。结果表明，来自每个来源的蛋白质具有相同的电泳迁移率，并且在免疫学上是相同的。通过凝胶过滤法估算分子大小，结果显示雏鸡小肠和母鸡子宫钙结合蛋白的分子量均约为28,000道尔顿。雏鸡小肠和母鸡子宫钙结合蛋白的氨基酸组成基本相同。