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来自葡萄糖 -1- C -14的C -14在汉逊德巴利酵母脂质组分中的分布。

Distribution of C-14 from glucose-1-C-14 in the lipid fractions of Debaryomyces hansenii.

作者信息

Merdinger E, Frye R H

出版信息

J Bacteriol. 1966 May;91(5):1831-3. doi: 10.1128/jb.91.5.1831-1833.1966.

Abstract

Merdinger, Emanuel (Roosevelt University, Chicago, Ill.), and Rosalind H. Frye. Distribution of C(14) from glucose-1-C(14) in the lipid fractions of Debaryomyces hansenii. J. Bacteriol. 91:1831-1833. 1966.-Debaryomyces hansenii cells were grown in a medium containing yeast extract, malt extract, glucose, sodium chloride, and nutrient salts, to which glucose-1-C(14) was added. The lipids extracted from the cells were fractionated by use of a single column packed with silicic acid. Of the total C(14) added to the culture medium, the neutral lipid fractions contained 21.06% while the phospholipid portions contained only 0.89%. The highest amount of C(14) among the neutral lipids was found in the fraction containing the hydrocarbons (11.64%). Among the phospholipids, the highest amount (0.66%) was found in phosphatidyl serine and phosphatidyl ethanolamine.

摘要

默丁格,伊曼纽尔(伊利诺伊州芝加哥罗斯福大学)和罗莎琳德·H·弗莱。汉逊德巴利酵母脂质部分中来自葡萄糖 -1-¹⁴C 的¹⁴C 分布。《细菌学杂志》91:1831 - 1833。1966 年。——汉逊德巴利酵母细胞在含有酵母提取物、麦芽提取物、葡萄糖、氯化钠和营养盐的培养基中生长,并添加葡萄糖 -1-¹⁴C。从细胞中提取的脂质通过使用填充有硅酸的单一柱进行分离。添加到培养基中的总¹⁴C 中,中性脂质部分含有 21.06%,而磷脂部分仅含有 0.89%。中性脂质中¹⁴C 含量最高的是含有烃类的部分(11.64%)。在磷脂中,磷脂酰丝氨酸和磷脂酰乙醇胺中¹⁴C 含量最高(0.66%)。

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