出芽短梗霉对葡萄糖 -l-14C 的摄取
Uptake of glucose-l-14C by Pullularia pullulans.
作者信息
Merdinger E
出版信息
J Bacteriol. 1969 Jun;98(3):1021-5. doi: 10.1128/jb.98.3.1021-1025.1969.
Pullularia pullulans cells were grown in a medium containing yeast extract, malt extract, glucose, and nutrient salts, in addition to glucose-1-(14)C. The lipids extracted from the cells were fractionated by use of a single column packed with silicic acid. Of the total radioactive carbon added to the culture medium, the neutral lipid fractions contained 24.8%, whereas the phospholipid portions contained only 2.1%. The largest amount (16.5%) of (14)C among the neutral lipids was found in the fraction containing the free sterols. Among the phospholipids, the largest amount (1.1%) was found in phosphatidylserine and phosphatidylethanolamine. The second largest amount (9.6%) of the total (14)C used was found in trehalose, followed by carbon dioxide (7.3%).
出芽短梗霉细胞在含有酵母提取物、麦芽提取物、葡萄糖、营养盐以及葡萄糖-1-(14)C的培养基中生长。从细胞中提取的脂质通过使用填充有硅酸的单一柱进行分离。添加到培养基中的总放射性碳中，中性脂质部分占24.8%，而磷脂部分仅占2.1%。在中性脂质中，(14)C含量最高的部分（16.5%）存在于含有游离甾醇的部分中。在磷脂中，磷脂酰丝氨酸和磷脂酰乙醇胺中的含量最高（1.1%）。所用总(14)C中第二高的含量（9.6%）存在于海藻糖中，其次是二氧化碳（7.3%）。
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