乙醇代谢对大鼠肝脏切片乳酸/丙酮酸比值的影响。
Influence of the metabolism of ethanol on the lactate/pyruvate ratio of rat-liver slices.
作者信息
Forsander O A
出版信息
Biochem J. 1966 Jan;98(1):244-7. doi: 10.1042/bj0980244.
Abstract
- The influence of ethanol on the redox level of the redox pair lactate/pyruvate has been studied in experiments with rat-liver slices. 2. Ethanol had no effect on oxygen consumption but strongly depressed carbon dioxide formation. On the assumption that ethanol is oxidized to acetate in the liver slices, it could be calculated that most of the oxygen that disappeared was consumed in this reaction. 3. Addition of ethanol to the incubation medium increased the lactate/pyruvate ratio and when all the ethanol had been oxidized the redox value decreased to the normal again. Ethanol depressed the pyruvate concentration, whereas the lactate concentration was not much influenced. 4. Acetaldehyde in the concentrations present during ethanol oxidation did not influence the lactate/pyruvate ratio. Higher concentrations, however, increased the redox state. 5. Acetate in the concentrations present during ethanol oxidation in the experiments, and also in higher concentrations, did not influence the lactate/pyruvate ratio. 6. The mechanism by which ethanol influences the lactate/pyruvate ratio is discussed.
摘要
- 在大鼠肝脏切片实验中研究了乙醇对氧化还原对乳酸盐/丙酮酸盐氧化还原水平的影响。2. 乙醇对氧气消耗没有影响，但强烈抑制二氧化碳生成。假设乙醇在肝脏切片中被氧化为乙酸盐，可以计算出消失的大部分氧气在该反应中被消耗。3. 向孵育培养基中添加乙醇会增加乳酸盐/丙酮酸盐的比率，当所有乙醇被氧化后，氧化还原值会再次降至正常。乙醇降低了丙酮酸盐浓度，而乳酸盐浓度受影响不大。4. 乙醇氧化过程中存在的乙醛浓度对乳酸盐/丙酮酸盐比率没有影响。然而，更高的浓度会增加氧化还原状态。5. 实验中乙醇氧化过程中存在的乙酸盐浓度以及更高浓度的乙酸盐都不会影响乳酸盐/丙酮酸盐比率。6. 讨论了乙醇影响乳酸盐/丙酮酸盐比率的机制。
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