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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Figueroa E, Vallejos R, Pfeifer A, Kahler C

Biochem J. 1966 Jan;98(1):253-9. doi: 10.1042/bj0980253.

Glycogen synthesized by rat-liver slices 0.5mm. thick incubated at 1atm. oxygen pressure in Hastings medium with glucose was localized in the cells of the periphery of the slice. Cells of the interior of this slice do not synthesize glycogen. 2. Inner cells of thin slices (about 0.3mm. thick) can synthesize glycogen when such slices are incubated under the same conditions, but oxygen pressures higher than 1atm. are required if inner cells of slices 0.5mm. or more thick are to be able to synthesize glycogen. 3. Localization of newly synthesized glycogen in rat-liver slices incubated in Hastings medium with glucose does not depend on glucose concentration. 4. Calculation of the minimum oxygen pressure required to synthesize glycogen gives values between 0.09 and 0.17atm. 5. The advantages of high oxygen pressures for the study of the synthesis of glycogen and other compounds that require ATP are discussed.

在哈斯汀斯培养基中，于1个大气压氧气压力下，用葡萄糖孵育的0.5毫米厚大鼠肝脏切片合成的糖原定位于切片周边的细胞中。该切片内部的细胞不合成糖原。2. 当薄切片（约0.3毫米厚）在相同条件下孵育时，其内部细胞可以合成糖原，但如果要使0.5毫米或更厚切片的内部细胞能够合成糖原，则需要高于1个大气压的氧气压力。3. 在含葡萄糖的哈斯汀斯培养基中孵育的大鼠肝脏切片中新合成糖原的定位不依赖于葡萄糖浓度。4. 合成糖原所需最低氧气压力的计算得出的值在0.09至0.17个大气压之间。5. 讨论了高氧气压力在研究糖原及其他需要ATP的化合物合成方面的优势。

Figueroa E, Vallejos R, Pfeifer A, Kahler C

Biochem J. 1966 Jan;98(1):253-9. doi: 10.1042/bj0980253.

Glycogen synthesized by rat-liver slices 0.5mm. thick incubated at 1atm. oxygen pressure in Hastings medium with glucose was localized in the cells of the periphery of the slice. Cells of the interior of this slice do not synthesize glycogen. 2. Inner cells of thin slices (about 0.3mm. thick) can synthesize glycogen when such slices are incubated under the same conditions, but oxygen pressures higher than 1atm. are required if inner cells of slices 0.5mm. or more thick are to be able to synthesize glycogen. 3. Localization of newly synthesized glycogen in rat-liver slices incubated in Hastings medium with glucose does not depend on glucose concentration. 4. Calculation of the minimum oxygen pressure required to synthesize glycogen gives values between 0.09 and 0.17atm. 5. The advantages of high oxygen pressures for the study of the synthesis of glycogen and other compounds that require ATP are discussed.

在哈斯汀斯培养基中，于1个大气压氧气压力下，用葡萄糖孵育的0.5毫米厚大鼠肝脏切片合成的糖原定位于切片周边的细胞中。该切片内部的细胞不合成糖原。2. 当薄切片（约0.3毫米厚）在相同条件下孵育时，其内部细胞可以合成糖原，但如果要使0.5毫米或更厚切片的内部细胞能够合成糖原，则需要高于1个大气压的氧气压力。3. 在含葡萄糖的哈斯汀斯培养基中孵育的大鼠肝脏切片中新合成糖原的定位不依赖于葡萄糖浓度。4. 合成糖原所需最低氧气压力的计算得出的值在0.09至0.17个大气压之间。5. 讨论了高氧气压力在研究糖原及其他需要ATP的化合物合成方面的优势。