胸腺嘧啶核苷和胸腺嘧啶脱氧核苷酸的选择性降解。
Selective degradation of thymidine and thymine deoxynucleotides.
作者信息
Burton K, Riley W T
出版信息
Biochem J. 1966 Jan;98(1):70-7. doi: 10.1042/bj0980070.
Abstract
- Osmium tetroxide in dilute ammonia oxidizes various pyrimidine nucleosides at different rates. Thymidine is oxidized about 45 times as fast as deoxycytidine. The phosphate groups may be eliminated from oxidized thymine nucleotides by successive treatments with alkali and then with diphenylamine in aqueous formic acid. The reactions can be applied to the selective degradation of thymidine in oligodeoxynucleotides.
摘要
- 四氧化锇在稀氨水中以不同速率氧化各种嘧啶核苷。胸苷的氧化速度约为脱氧胞苷的45倍。通过用碱然后用甲酸水溶液中的二苯胺连续处理，可以从氧化的胸腺嘧啶核苷酸中除去磷酸基团。这些反应可用于寡脱氧核苷酸中胸苷的选择性降解。
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