Free fatty acids and lipolytic activity of the pancreas of irradiated rats.

The effect of X-ray irradiation (800 R) on the lipolytic activity of pancreas was studied in rats. Lipase activity was assayed in the granules of zymogenmitochrondial fraction and in the perfusion fluid and free fatty acids (FFA) content was determined in blood serum, pancreatic homogenates and in perfusion fluid. The irradiation caused a marked increase of the activity of lipase released from zymogen-mitochondrial fraction. In the perfusate the enhancement of lipase activity accompanied by a simultaneous increase of FFA content. Irradiation resulted in an increase of FFA in pancreatic homogenates and a decrease of their content in blood serum.