Bussard A E, Hannoun C
J Exp Med. 1966 Jun 1;123(6):1047-60. doi: 10.1084/jem.123.6.1047.
By combining a tissue culture method with the detection of antibody-producing cells by local hemolysis in gum it has been possible to follow the immunological activity of cells from tissue fragments for long period of time. These fragments were obtained from lymph nodes or spleens of rabbits immunized by sheep erythrocytes. It was found that, while the immunological activity of the free cells in suspensions decreased fast and disappeared in a few days, the cells attaching on glass could express their activity for at least 3 wk. It is assumed that these cells are the daughters of cells from the fragments which were not active antibody producers at the beginning, but differentiated, during the culture, into cells endowed with two capacities: glass adherence and antibody synthesis. One can further admit that the type of culture employed exerts a selective pressure favoring formation of antibody-producing cells.
通过将组织培养方法与通过牙龈局部溶血检测抗体产生细胞相结合，得以长时间追踪来自组织碎片的细胞的免疫活性。这些碎片取自经绵羊红细胞免疫的兔子的淋巴结或脾脏。结果发现，虽然悬浮液中游离细胞的免疫活性迅速下降并在几天内消失，但附着在玻璃上的细胞至少可在3周内保持其活性。据推测，这些细胞是最初并非活跃抗体产生者的碎片细胞的子代，它们在培养过程中分化为具有两种能力的细胞：玻璃黏附能力和抗体合成能力。还可以进一步认为，所采用的培养类型施加了有利于抗体产生细胞形成的选择压力。
