Hannoun C, Bussard A E
J Exp Med. 1966 Jun 1;123(6):1035-46. doi: 10.1084/jem.123.6.1035.
An organ culture technique was used to investigate the migration and the morphological evolution of lymphocytes from lymphopoietic tissues. This evolution was compared with the behavior of cells extracted from the tissue and kept in nutritive medium in vitro. It was found that cells were continuously migrating from the fragments of lymph nodes or spleen, and were attaching to the glass. They spread on glass, their protoplasm enlarged and their nucleus became clearer. The evolution towards blastoid cells was identical with that described under artificial stimulation by PHA for example. Cytological identification of the cells actively engaged in antibody synthesis (as detected by local hemolysis in gum) at the time of staining, showed that several distinct cellular types were active, including plasma cells and macrophagelike cells. It is assumed that the stimulated lymphocytes, after spontaneous migration from the tissue are able to evolve into an "immunoblast" stage and then, eventually after fixation upon a physical support, to initiate antibody synthesis.
采用器官培养技术研究淋巴细胞从淋巴组织的迁移及形态演变。将这种演变与从组织中提取并置于体外营养培养基中的细胞行为进行比较。发现细胞不断从淋巴结或脾脏碎片中迁移出来，并附着在玻璃上。它们在玻璃上铺展，原生质增大，细胞核变得更清晰。向母细胞样细胞的演变与例如在PHA人工刺激下所描述的相同。染色时对积极参与抗体合成的细胞（通过牙龈局部溶血检测）进行细胞学鉴定，结果表明有几种不同的细胞类型活跃，包括浆细胞和巨噬细胞样细胞。据推测，受刺激的淋巴细胞从组织自发迁移后，能够演变成“免疫母细胞”阶段，然后最终在附着于物理支持物后启动抗体合成。
