圆柱鱼腥藻亚细胞制剂对硝酸盐和亚硝酸盐的还原作用。I. 亚硝酸盐还原为氨
Reduction of nitrate and nitrite by subcellular preparations of Anabaena cylindrica. I. Reduction of nitrite to ammonia.
作者信息
Hattori A, Myers J
出版信息
Plant Physiol. 1966 Jun;41(6):1031-6. doi: 10.1104/pp.41.6.1031.
Abstract
Reduction of nitrite by cell-free preparations of Anabaena cylindrica in the dark has been investigated. Nitrite-reducing activity was recovered in a supernatant fraction. The nitrite reductase system was partially purified by column chromatography on Sephadex G-75. NADPH could serve as an H-donor. NADH was completely inactive. The reduction required ferredoxin which mediated the transfer of electrons from NADPH to nitrite. Ferredoxin was successfully replaced with methyl viologen, benzyl viologen and diquat. The nitrite-reducing activity was inhibited by KCN, and by 2,4-dinitrophenol and arsenate at higher concentrations. The extent of nitrite reduction by NADPH was dependent on the oxidation-reduction states of NADP and ferredoxin.
摘要
已对黑暗条件下圆柱鱼腥藻无细胞制剂还原亚硝酸盐的情况进行了研究。亚硝酸盐还原活性在一个上清液组分中得以恢复。亚硝酸盐还原酶系统通过在葡聚糖凝胶G - 75上进行柱色谱法进行了部分纯化。NADPH可作为氢供体。NADH完全无活性。该还原反应需要铁氧还蛋白，它介导电子从NADPH转移至亚硝酸盐。铁氧还蛋白成功地被甲基紫精、苄基紫精和百草枯替代。亚硝酸盐还原活性受到KCN以及较高浓度的2,4 - 二硝基苯酚和砷酸盐的抑制。NADPH还原亚硝酸盐的程度取决于NADP和铁氧还蛋白的氧化还原状态。
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本文引用的文献
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