粗糙脉孢菌对亚硝酸盐的摄取。
Uptake of nitrite by Neurospora crassa.
作者信息
Schloemer R H, Garrett R H
出版信息
J Bacteriol. 1974 Apr;118(1):270-4. doi: 10.1128/jb.118.1.270-274.1974.
Abstract
Like the nitrate transport system, the nitrite uptake system in Neurospora crassa is induced by either nitrate or nitrite. This induction is prevented by cycloheximide, puromycin, or 6-methyl purine. The K(m) for nitrite of the induced nitrite uptake system is 86 muM, and the V(max) is 100 mumol of nitrite per g (wet weight) per h. Nitrite uptake is inhibited by metabolic poisons such as arsenate, dinitrophenol, cyanide, and antimycin A. No repression or inhibition of the nitrite transport system by ammonia, nitrate, or Casamino Acids was observed.
摘要
与硝酸盐转运系统一样，粗糙脉孢菌中的亚硝酸盐摄取系统可由硝酸盐或亚硝酸盐诱导。这种诱导可被环己酰亚胺、嘌呤霉素或6-甲基嘌呤阻止。诱导的亚硝酸盐摄取系统对亚硝酸盐的米氏常数（K(m)）为86微摩尔，最大反应速度（V(max)）为每克（湿重）每小时100微摩尔亚硝酸盐。亚硝酸盐摄取受到代谢毒物如砷酸盐、二硝基苯酚、氰化物和抗霉素A的抑制。未观察到氨、硝酸盐或酪蛋白氨基酸对亚硝酸盐转运系统的阻遏或抑制作用。
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