Simionescu L, Oprescu M, Ghinea E, Ghinea L, Săhleanu V, Dimitriu V
Endocrinologie. 1977 Oct-Dec;15(4):243-8.
A radioimmunoassay (RIA) technique of human thyroglobulin (Tgl) was developed using 125I-Tgl and a rabbit anti-Tgl serum at the initial dilution of 1:100,000. The sensitivity of the technique was of 2.5 ng/ml. The RIA of Tgl was applied as a screening test for the in vitro secretory activity of human thyroid cells under the influence of two herbicides of the heterocyclic group (Amitrol and Atrazin). Three levels of concentration of Amitrol and Atrazin were administered to the tissue culture tubes containing in the first series of experiments perinodular tissue (Typ), and in the second one nodular tissue (TyN). The thyroid gland was surgically obtained from a patient with the diagnosis of polynodular goiter. Only the high levels (10 mg and 1.0 mg/tube, respectively) of herbicide concentration inhibited the secretion and release of Tgl, the nodular tissue being more resistant to the toxic. However, parallel studies including the dosage of the active hormones (T3, T4) are necessary in order to appreciate the value of Tgl determination as an indicator of hormogenesis in this type of experiments.
利用125I-甲状腺球蛋白（Tgl）和初始稀释度为1:100,000的兔抗Tgl血清，开发了一种人甲状腺球蛋白（Tgl）的放射免疫分析（RIA）技术。该技术的灵敏度为2.5 ng/ml。Tgl的RIA被用作一种筛选试验，用于检测在两种杂环类除草剂（灭草隆和莠去津）影响下人甲状腺细胞的体外分泌活性。在第一组实验中，将三种浓度水平的灭草隆和莠去津施用于含有甲状腺结节周围组织（Typ）的组织培养管，在第二组实验中施用于含有甲状腺结节组织（TyN）的组织培养管。甲状腺取自一名诊断为多结节性甲状腺肿的患者。只有高浓度水平（分别为10 mg和1.0 mg/管）的除草剂抑制了Tgl的分泌和释放，结节组织对毒性更具抗性。然而，为了评估在这类实验中Tgl测定作为激素生成指标的价值，还需要进行包括活性激素（T3、T4）剂量测定的平行研究。
