Gryz K, Jarzebiński J, Traczyk W Z, Wilmańska D
Acta Physiol Pol. 1977 Jul-Aug;28(4):383-9.
Using the method of Chang and Leeman for isolation the sialogogic undecapeptide from bovine hypothalamus partly purified Substance P was obtained. The final steps of purification were omitted to prevent losing of other peptides possessing biological activity of Substance P. The qualitative and quantitative content of amino acids in the hypothalamic extract was determined and fourteen amino acids were found. Four of them were not present in the sialogogic undecapeptide or neurotensin. It can be concluded that in the hypothalamic tissue apart from sialogogic undecapeptide and neurotensin, there are other peptides related to these two.
采用张和利曼的方法从牛下丘脑分离促唾液分泌的十一肽，得到了部分纯化的P物质。为防止失去具有P物质生物活性的其他肽，省略了纯化的最后步骤。测定了下丘脑提取物中氨基酸的定性和定量含量，发现了14种氨基酸。其中四种不存在于促唾液分泌的十一肽或神经降压素中。可以得出结论，在下丘脑组织中，除了促唾液分泌的十一肽和神经降压素外，还有其他与这两种肽相关的肽。
