Hammer R A, Leeman S E, Carraway R, Williams R H
J Biol Chem. 1980 Mar 25;255(6):2476-80.
The biologically active peptide neurotensin (NT) has been isolated from fresh postmortem human small intestine and its identity with bovine hypothalamic and intestinal neurotensin has been established. Purification was achieved by gel filtration and two ion exchange chromatography steps; material was detected by radioimmunoassay (RIA). A substance was obtained that had integral molar ratios of amino acids and eluted in a single peak during reverse-phase high pressure liquid chromatography (HPLC). This material had an amino acid composition and COOH-terminal sequence identical with those of bovine NT. Human and bovine neurotensin gave rise to the same fragments when treated with papain; they were indistinguishable in RIAs using three different region-specific antisera and in their hypotensive effect on anesthetized rats. Using mucosal scrapings obtained immediately post-mortem from four subjects, the concentration of immunoreactive neurotensin was found to increase from duodenum to distal ileum, in agreement with results obtained in other mammalian species.
生物活性肽神经降压素（NT）已从新鲜的人死后小肠中分离出来，并确定其与牛下丘脑和肠道神经降压素相同。通过凝胶过滤和两步离子交换色谱法实现了纯化；通过放射免疫测定法（RIA）检测物质。获得了一种具有氨基酸整体摩尔比且在反相高压液相色谱（HPLC）中以单峰洗脱的物质。该物质的氨基酸组成和羧基末端序列与牛NT相同。人源和牛源神经降压素在用木瓜蛋白酶处理时产生相同的片段；在使用三种不同区域特异性抗血清的放射免疫测定中以及对麻醉大鼠的降压作用方面，它们无法区分。使用从四名受试者死后立即获得的黏膜刮片，发现免疫反应性神经降压素的浓度从十二指肠到回肠末端增加，这与在其他哺乳动物物种中获得的结果一致。
