马抗体肽链的抗原结合能力。
The antigen-binding capacity of the peptide chains of horse antibodies.
作者信息
Weir R C, Porter R R
出版信息
Biochem J. 1966 Jul;100(1):69-72. doi: 10.1042/bj1000069.
Abstract
The antigen-binding capacity of the peptide chains of horse anti-(diphtheria toxin) has been studied by using (125)I-labelled toxoid and electrophoresis of antibody-antigen mixtures on cellulose acetate. The heavy chains retained about 20% of the activity of the whole antibody and the light chains less than 5%. Recombination of specific heavy and light chains gave about 60% recovery of activity and recombination of specific heavy chains and non-specific light chains about 40% recovery. It is suggested that these results favour the heavy chain as the major site of the antigen-binding activity.
摘要
通过使用（125）I标记的类毒素以及在醋酸纤维素上对抗体-抗原混合物进行电泳，研究了马抗（白喉毒素）肽链的抗原结合能力。重链保留了整个抗体约20%的活性，轻链保留的活性不到5%。特异性重链和轻链的重组使活性恢复约60%，特异性重链和非特异性轻链的重组使活性恢复约40%。这些结果表明，重链是抗原结合活性的主要位点。
相似文献
1
The antigen-binding capacity of the peptide chains of horse antibodies.马抗体肽链的抗原结合能力。
Biochem J. 1966 Jul;100(1):69-72. doi: 10.1042/bj1000069.
2
[Heterogeneity of horse antitetanus and antidiphtheria light chains].[马抗破伤风和抗白喉轻链的异质性]
Prog Immunobiol Stand. 1970;4:31-7.
3
Comparison of the structure of the immunoglobulins from horse serum.马血清免疫球蛋白结构的比较。
Biochem J. 1966 Jul;100(1):63-8. doi: 10.1042/bj1000063.
4
[Preparation and properties of horse alpha2-macroglobulin].[马α2-巨球蛋白的制备与性质]
Ann Inst Pasteur (Paris). 1970 Jul;119(1):27-49.
5
Evaluation of light chain antigen binding by radioimmunoelectrophoresis.通过放射免疫电泳评估轻链抗原结合情况。
J Immunol. 1967 Sep;99(3):590-6.
6
[Antibody (anti-diptheria) activity in the light chain preparations].
Bull Soc Chim Biol (Paris). 1968 Sep 28;50(5):1041-8.
7
[Isolation of anti-tetanus antibodies from diatherm-3 serum on immunosorbents].
Zh Mikrobiol Epidemiol Immunobiol. 1968 Mar;45(3):124-7.
8
[Antibody activity in light chain preparations].
Ann Inst Pasteur (Paris). 1967 Oct;113(4):549-67.
9
Recovery of antibody activity from inactive hybrids of H and L chains.从无活性的重链和轻链杂交体中恢复抗体活性。
J Exp Med. 1967 Jan 1;125(1):191-7. doi: 10.1084/jem.125.1.191.
10
MULTIPLICITY OF ANTIBODY PROTEINS IN RABBIT ANTI-P-AZOBENZENEARSONATE SERA.兔抗对氨基苯砷酸血清中抗体蛋白的多样性
J Immunol. 1964 Feb;92:173-84.
引用本文的文献
1
Biliary excretion in foreign compounds. Species difference in biliary excretion.外来化合物的胆汁排泄。胆汁排泄的种属差异。
Biochem J. 1967 Dec;105(3):1289-93. doi: 10.1042/bj1051289.
2
Biliary excretion of foreign compounds. Biphenyl, stilboestrol and phenolphthalein in the rat: molecular weight, polarity and metabolism as factors in biliary excretion.外源性化合物经胆汁排泄。二苯、己烯雌酚和酚酞在大鼠体内的胆汁排泄：分子量、极性和代谢作为胆汁排泄的因素。
Biochem J. 1967 Dec;105(3):1275-81. doi: 10.1042/bj1051275.
3
The role of gut flora in the enterohepatic circulation of stilboestrol in the rat.肠道菌群在大鼠己烯雌酚肝肠循环中的作用。
Biochem J. 1969 Mar;112(1):17P-18P. doi: 10.1042/bj1120017pb.
4
Immunoglobulins and immunocytes.免疫球蛋白与免疫细胞。
Bacteriol Rev. 1969 Jun;33(2):159-71. doi: 10.1128/br.33.2.159-171.1969.
5
The structure of the heavy chain of immunoglobulin and its relevance to the nature of the antibody-combining site. The Second CIBA Medal Lecture.免疫球蛋白重链的结构及其与抗体结合位点性质的相关性。第二届CIBA奖章讲座。
Biochem J. 1967 Nov;105(2):417-26. doi: 10.1042/bj1050417.
6
The N-terminal sequence of the heavy chain of rabbit immunoglobulin IgG.兔免疫球蛋白IgG重链的N端序列。
Biochem J. 1966 Aug;100(2):303-8. doi: 10.1042/bj1000303.
7
Proceedings: The enterohepatic circulation of 3H-phenolphthalein in the rat.论文：大鼠体内3H-酚酞的肠肝循环
Br J Pharmacol. 1976 Mar;56(3):355P-356P.
8
Distribution of injected 14C-diethylstilbestrol in the chicken and laying hen. Whole body autoradiography and impulse counting.注射的14C - 己烯雌酚在鸡和产蛋母鸡体内的分布。全身放射自显影和脉冲计数。
Acta Vet Scand. 1978;19(2):254-62. doi: 10.1186/BF03547630.
9
Comparison of the novel bibenzyl bifluranol with diethystilboestrol: effect of aromatic fluorine substitution on metabolism [proceedings].
Br J Pharmacol. 1978 Jun;63(2):350P.
本文引用的文献
1
RECOVERY OF SPECIFIC ACTIVITY AFTER COMPLETE UNFOLDING AND REDUCTION OF AN ANTIBODY FRAGMENT.抗体片段完全展开和还原后比活性的恢复
Proc Natl Acad Sci U S A. 1965 Mar;53(3):524-32. doi: 10.1073/pnas.53.3.524.
2
STRUCTURE AND BIOLOGICAL ACTIVITY OF IMMUNOGLOBULINS.免疫球蛋白的结构与生物活性
Adv Immunol. 1964;27:287-349. doi: 10.1016/s0065-2776(08)60710-5.
3
POLYPEPTIDE CHAINS OF ANTIBODY: EFFECTIVE BINDING SITES REQUIRE SPECIFICITY IN COMBINATION.抗体的多肽链：有效的结合位点需要结合特异性。
Science. 1965 Feb 5;147(3658):613-5. doi: 10.1126/science.147.3658.613.
4
RECOVERY OF ANTIGENIC SPECIFICITY AFTER DENATURATION AND COMPLETE REDUCTION OF DISULFIDES IN A PAPAIN FRAGMENT OF ANTIBODY.抗体木瓜蛋白酶片段中变性及二硫键完全还原后抗原特异性的恢复
Proc Natl Acad Sci U S A. 1964 Oct;52(4):1099-106. doi: 10.1073/pnas.52.4.1099.
5
SPECIFIC COMBINATION OF H AND L CHAINS OF RABBIT GAMMA-GLOBULINS.兔γ球蛋白H链和L链的特定组合
Proc Natl Acad Sci U S A. 1964 Feb;51(2):173-8. doi: 10.1073/pnas.51.2.173.
6
RECONSTITUTION OF IMMUNOLOGIC ACTIVITY BY INTERACTION OF POLYPEPTIDE CHAINS OF ANTIBODIES.通过抗体多肽链相互作用重建免疫活性
Proc Natl Acad Sci U S A. 1963 Oct;50(4):753-61. doi: 10.1073/pnas.50.4.753.
7
THE ARRANGEMENT OF THE PEPTIDE CHAINS IN GAMMA-GLOBULIN.γ球蛋白中肽链的排列
Biochem J. 1963 Aug;88(2):220-8. doi: 10.1042/bj0880220.
8
Recovery of antibody combining activity by interaction of different peptide chains isolated from purified horse antitoxins.通过从纯化的马抗毒素中分离出的不同肽链相互作用恢复抗体结合活性。
Folia Microbiol (Praha). 1963 Mar;8:128-30. doi: 10.1007/BF02877236.
9
Efficient trace-labelling of proteins with iodine.用碘对蛋白质进行高效的示踪标记。
Nature. 1958 Jul 5;182(4627):53. doi: 10.1038/182053a0.
10
Insulin-I131 metabolism in human subjects: demonstration of insulin binding globulin in the circulation of insulin treated subjects.人类受试者中胰岛素-I131的代谢：胰岛素治疗受试者循环中胰岛素结合球蛋白的证实。
J Clin Invest. 1956 Feb;35(2):170-90. doi: 10.1172/JCI103262.
Zotero 插件