兔免疫球蛋白IgG重链的N端序列。
The N-terminal sequence of the heavy chain of rabbit immunoglobulin IgG.
作者信息
Wilkinson J M, Press E M, Porter R R
出版信息
Biochem J. 1966 Aug;100(2):303-8. doi: 10.1042/bj1000303.
Abstract
The absence of an N-terminal amino acid with a free alpha-amino group from the heavy chain of rabbit immunoglobulin IgG has been confirmed and no evidence could be found of a blocking formyl, acetyl or propionyl group. The N-terminal amino acid appears to be pyrrolid-2-one-5-carboxylic acid (PCA) in all molecules. A mixed amino acid sequence follows in the approximate proportions: PCA-Ser-Val-Glu-Glu-Ser-Gly-Gly-Arg, 50%; PCA-Ser-Leu-Glu, 20%; PCA-Glu(NH(2)), 20%. The heavy chains of a purified antibody, namely anti-(human serum albumin), and of immunoglobulin IgG from a rabbit homozygous at the allotypic loci both showed a similar mixed N-terminal sequence.
摘要
已证实兔免疫球蛋白IgG重链不存在带有游离α-氨基的N端氨基酸，且未发现有封闭的甲酰基、乙酰基或丙酰基的证据。在所有分子中，N端氨基酸似乎都是吡咯烷-2-酮-5-羧酸（PCA）。随后是一个混合氨基酸序列，其大致比例为：PCA-丝氨酸-缬氨酸-谷氨酸-谷氨酸-丝氨酸-甘氨酸-甘氨酸-精氨酸，50%；PCA-丝氨酸-亮氨酸-谷氨酸，20%；PCA-谷氨酸（NH(2)），20%。纯化抗体即抗（人血清白蛋白）的重链以及来自同种异型位点纯合兔的免疫球蛋白IgG都显示出类似的混合N端序列。
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